extractionofsolublecollagenanditsfeasibilityinthepalaeodietaryresearch

IVPP-IR

	extractionofsolublecollagenanditsfeasibilityinthepalaeodietaryresearch
	Wang Ning1 ; Hu Yaowu 1; Hou Liangliang 2; Yang Ruiping 1; Song Guoding 1; Wang Changsui 1
	2014
发表期刊	sciencechinaearthsciences
ISSN	1674-7313
卷号	57 期号:5 页码:1039
摘要	In current palaeodietary research, gelatinization is the main method to extract insoluble collagen(ISC) from ancient bones. However, the degradation products of ISC, i.e., soluble collagen(SC), is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be attributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ~(13)C and δ~(15)N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range(2.9–3.6), the mean difference of δ~(13)C value was only(0.3±0.2)‰(n=2) while δ~(15)N value was(0.6±0.1)‰(n=2). Although the atomic C/N ratios of some SC are slightly beyond the normal range, the mean differences of δ~(13)C and δ~(15)N values were still only(0.4±0.1)‰ and(0.3±0)‰(n=2) respectively. These isotopic differences are quite below the isotope fractionation in one trophic level(δ~(13)C values of 1‰–1.5‰ and δ~(15)N values of 3‰–5‰), suggesting that SC had great application potentials in palaeodietary research.
语种	英语
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/12996
专题	中国科学院古脊椎动物与古人类研究所
作者单位	1.中国科学院古脊椎动物与古人类研究所 2.山西大学
第一作者单位	中国科学院古脊椎动物与古人类研究所
推荐引用方式 GB/T 7714	Wang Ning,Hu Yaowu,Hou Liangliang,et al. extractionofsolublecollagenanditsfeasibilityinthepalaeodietaryresearch[J]. sciencechinaearthsciences,2014,57(5):1039.
APA	Wang Ning,Hu Yaowu,Hou Liangliang,Yang Ruiping,Song Guoding,&Wang Changsui.(2014).extractionofsolublecollagenanditsfeasibilityinthepalaeodietaryresearch.sciencechinaearthsciences,57(5),1039.
MLA	Wang Ning,et al."extractionofsolublecollagenanditsfeasibilityinthepalaeodietaryresearch".sciencechinaearthsciences 57.5(2014):1039.