DNA staining in fossil cells beyond the Quaternary: Reassessment of the evidence and prospects for an improved understanding of DNA preservation in deep time

doi:10.1016/j.earscirev.2021.103600

IVPP-IR > 中科院古脊椎所（2000年以后）

	DNA staining in fossil cells beyond the Quaternary: Reassessment of the evidence and prospects for an improved understanding of DNA preservation in deep time
	Bailleul, Alida M.1,2; Li, Zhiheng1,2
	2021-05-01
发表期刊	EARTH-SCIENCE REVIEWS
ISSN	0012-8252
卷号	216 页码:11
通讯作者	Bailleul, Alida M.(alida.bailleul@ivpp.ac)
摘要	The discovery in the 1980?s that DNA could be extracted and sequenced from extinct animals opened-up a whole new area of research in paleobiology. The oldest authenticated sequence ever recovered is between 1.65 and 1.1 million years (My) old and extrapolation models on DNA degradation suggest that this is close to the temporal limit of DNA survival. However, recent data from cell nuclei in 30 to 80 My-old fossil cells from plants and dinosaurs show positive staining with standard DNA stains and fluorochromes. We heavily discuss and scrutinize the results of these studies and argue that the intracellular stainings seen in these Cenozoic and Cretaceous fossils are consistent with the presence of endogenous DNA and inconsistent with contamination. Properties of the stains suggest that the preserved molecules are made of at least the sugar-phosphate backbone of DNA, and in some instances they may be double stranded with preserved base pairs. Previous works on DNA damage also suggest that the material is crosslinked and filled with chemically modified nucleotides, which may explain why it is apparently not in a PCR-amplifiable nor in a sequenceable form. However, even though many questions remain, it cannot yet be ruled out that retrieving ancient sequences in fossils older than the Pleistocene will be possible in the future. Here, we summarize and reassess all current evidence and propose new methods and ideas on how to further understand DNA preservation in deep time. Notably, microscopy-based DNA sequencing may offer the most promising results. The main goal of this review is to show the need for new collaborations between the fields of Ancient DNA, Molecular Paleontology and Paleohistology to better seek the truth about DNA fossilization.
关键词	Fossil cells Nuclei Histochemistry Cytochemistry DNA staining DNA sequencing
DOI	10.1016/j.earscirev.2021.103600
关键词[WOS]	NUCLEIC-ACIDS ; ANCIENT DNA ; SEQUENCE ; BONE ; RECORD ; BIOMOLECULES ; CHROMOSOMES ; OSTEOCYTES ; PROTEINS ; ARCHAEAL
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41688103] ; CAS-PIFI (Chinese Academy of Sciences-President's International Fellowship Initiative)
项目资助者	National Natural Science Foundation of China ; CAS-PIFI (Chinese Academy of Sciences-President's International Fellowship Initiative)
WOS研究方向	Geology
WOS类目	Geosciences, Multidisciplinary
WOS记录号	WOS:000641464100003
出版者	ELSEVIER
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/18427
专题	中科院古脊椎所（2000年以后）
通讯作者	Bailleul, Alida M.
作者单位	1.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing 100044, Peoples R China 2.CAS Ctr Excellence Life & Paleoenvironm, Beijing 100044, Peoples R China
推荐引用方式 GB/T 7714	Bailleul, Alida M.,Li, Zhiheng. DNA staining in fossil cells beyond the Quaternary: Reassessment of the evidence and prospects for an improved understanding of DNA preservation in deep time[J]. EARTH-SCIENCE REVIEWS,2021,216:11.
APA	Bailleul, Alida M.,&Li, Zhiheng.(2021).DNA staining in fossil cells beyond the Quaternary: Reassessment of the evidence and prospects for an improved understanding of DNA preservation in deep time.EARTH-SCIENCE REVIEWS,216,11.
MLA	Bailleul, Alida M.,et al."DNA staining in fossil cells beyond the Quaternary: Reassessment of the evidence and prospects for an improved understanding of DNA preservation in deep time".EARTH-SCIENCE REVIEWS 216(2021):11.