DiceCT applied to fossilized hard tissues: A preliminary case study using a miocene bird

doi:10.1002/jez.b.23037

IVPP-IR > 中科院古脊椎所（2000年以后）

	DiceCT applied to fossilized hard tissues: A preliminary case study using a miocene bird
	Bailleul, Alida M.1,2; Lu, Jing 1,2; Li, Zhiheng1,2
	2021-03-05
发表期刊	JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
ISSN	1552-5007
页码	12
通讯作者	Bailleul, Alida M.(alida.bailleul@ivpp.ac.cn) ; Li, Zhiheng(lizhiheng@ivpp.ac.cn)
摘要	Iodine-based contrasting agents for computed tomography (CT) have been used for decades in medicine. Agents like Lugol's iodine enhance the contrasts between soft tissues and mineralized (skeletal) tissues. Because a recent study on extant avian heads showed that iodine-ethanol (I2E) is a better contrast enhancer overall than the standard Lugol's iodine, here, we tested if I2E could also enhance the CT contrasts of two fossilized skeletal tissues: bone and calcified cartilage. For this, we used a partial ankle joint from an extinct pheasant from the Late Miocene of Northwest China (Linxia Basin). The pre-staining CT scans showed no microstructural details of the sample. After being immersed into a solution of 1% I2E for 8 days and scanned a second time, the contrasts were drastically enhanced between the mineralized tissues (bony trabeculae and calcified cartilage) and the sediments and minerals inside vascular spaces. After three other staining-scanning cycles in 2%, 3%, and 6% I2E solutions, the best contrasts were obtained after immersion in 6% I2E for 7 days. Energy Dispersive Spectroscopy showed that iodine was preferentially absorbed by the mineralized tissues and the minerals in the vascular spaces, but not by the sediments. This method not only effectively increased the contrasts of two different fossilized skeletal tissues, it was also non-destructive and reversible because part of the fossil was successfully de-stained after a few days in pure ethanol. These preliminary results indicate that iodine-ethanol has the potential to be used widely in vertebrate paleontology to improve CT imaging of fossilized tissues.
关键词	computed tomography diceCT fossil bone fossil bird fossil cartilage iodine– ethanol miocene
DOI	10.1002/jez.b.23037
收录类别	SCI
语种	英语
资助项目	China National Science Foundation[41772013] ; One Hundred Talent Grant[KC17113] ; Strategic Priority Research Program of Chinese Academy of Sciences, XDA[19050100] ; Strategic Priority Research Program of Chinese Academy of Sciences, XDA[XDB26000000] ; Second Tibetan Plateau Scientific Expedition and Research Grant[2019QZKK0705] ; Craton destruction and terrestrial life evolution[41688103]
项目资助者	China National Science Foundation ; One Hundred Talent Grant ; Strategic Priority Research Program of Chinese Academy of Sciences, XDA ; Second Tibetan Plateau Scientific Expedition and Research Grant ; Craton destruction and terrestrial life evolution
WOS研究方向	Evolutionary Biology ; Developmental Biology ; Zoology
WOS类目	Evolutionary Biology ; Developmental Biology ; Zoology
WOS记录号	WOS:000625418000001
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/18643
专题	中科院古脊椎所（2000年以后）
通讯作者	Bailleul, Alida M.; Li, Zhiheng
作者单位	1.Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing 100044, Peoples R China 2.CAS Ctr Excellence Life & Paleoenvironm, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Bailleul, Alida M.,Lu, Jing,Li, Zhiheng. DiceCT applied to fossilized hard tissues: A preliminary case study using a miocene bird[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,2021:12.
APA	Bailleul, Alida M.,Lu, Jing,&Li, Zhiheng.(2021).DiceCT applied to fossilized hard tissues: A preliminary case study using a miocene bird.JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION,12.
MLA	Bailleul, Alida M.,et al."DiceCT applied to fossilized hard tissues: A preliminary case study using a miocene bird".JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION (2021):12.