| Extraction of soluble collagen and its feasibility in the palaeodietary research | |
| Wang Ning1,2; Hu YaoWu1,2; Hou LiangLiang3; Yang RuiPing1,2; Song GuoDing1,2; Wang ChangSui1,2; Hu, YW (reprint author), Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing 100044, Peoples R China. | |
| 2014-05-01 | |
| 发表期刊 | SCIENCE CHINA-EARTH SCIENCES
 |
| ISSN | 1674-7313 |
| 卷号 | 57期号:5页码:1039-1047 |
| 文章类型 | Article |
| 摘要 | In current palaeodietary research, gelatinization is the main method to extract insoluble collagen (ISC) from ancient bones. However, the degradation products of ISC, i.e., soluble collagen (SC), is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be attributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the delta C-13 and delta N-15 values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range (2.9-3.6), the mean difference of delta C-13 value was only (0.3 +/- 0.2)aEuro degrees (n=2) while delta N-15 value was (0.6 +/- 0.1)aEuro degrees (n=2). Although the atomic C/N ratios of some SC are slightly beyond the normal range, the mean differences of delta C-13 and delta N-15 values were still only (0.4 +/- 0.1)aEuro degrees and (0.3 +/- 0)aEuro degrees (n=2) respectively. These isotopic differences are quite below the isotope fractionation in one trophic level (delta C-13 values of 1aEuro degrees aEuro"1.5aEuro degrees and delta N-15 values of 3aEuro degrees aEuro"5aEuro degrees), suggesting that SC had great application potentials in palaeodietary research. |
| 关键词 | Soluble Collagen Insoluble Collagen Gel Chromatography Stable Isotopic Analysis |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 关键词[WOS] | INDIVIDUAL AMINO-ACIDS ; ISOTOPIC ANALYSIS ; BONE-COLLAGEN ; ARCHAEOLOGICAL BONE ; SURVIVAL ; NITROGEN ; CARBON ; PRESERVATION ; DIETARY ; SITE |
| 收录类别 | SCI |
| 语种 | 英语 |
| 项目资助者 | CAS Strategic Priority Research Program [XDA05130303]; Chinese Academy of Sciences & Max-Planck Institute Partnership Group Project [KACX1-YW-0830] |
| WOS研究方向 | Geology |
| WOS类目 | Geosciences, Multidisciplinary |
| WOS记录号 | WOS:000335244800018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.205/handle/311034/5164 |
| 专题 | 古人类及旧石器研究室 |
| 通讯作者 | Hu, YW (reprint author), Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing 100044, Peoples R China. |
| 作者单位 | 1.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing 100044, Peoples R China 2.Univ Chinese Acad Sci, Dept Sci Hist & Archaeometry, Beijing 100049, Peoples R China 3.Shanxi Univ, Sch Hist & Culture, Taiyuan 030006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang Ning,Hu YaoWu,Hou LiangLiang,et al. Extraction of soluble collagen and its feasibility in the palaeodietary research[J]. SCIENCE CHINA-EARTH SCIENCES,2014,57(5):1039-1047. |
| APA | Wang Ning.,Hu YaoWu.,Hou LiangLiang.,Yang RuiPing.,Song GuoDing.,...&Hu, YW .(2014).Extraction of soluble collagen and its feasibility in the palaeodietary research.SCIENCE CHINA-EARTH SCIENCES,57(5),1039-1047. |
| MLA | Wang Ning,et al."Extraction of soluble collagen and its feasibility in the palaeodietary research".SCIENCE CHINA-EARTH SCIENCES 57.5(2014):1039-1047. |
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