Proteomic evidence of dietary sources in ancient dental calculus

doi:10.1098/rspb.2018.0977

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	Proteomic evidence of dietary sources in ancient dental calculus
	Hendy, Jessica 1,3; Warinner, Christina 2,4,5,7; Bouwman, Abigail 5; Collins, Matthew J.3,8; Fiddyment, Sarah 3; Fischer, Roman 10; Hagan, Richard 2,4; Hofman, Courtney A.4; Holst, Malin 3,12; Chaves, Eros 7,13; Klaus, Lauren 4,7; Larson, Greger 11; Mackie, Meaghan 8,9; McGrath, Krista 3; Mundorff, Amy Z.14; Radini, Anita 3; Rao, Huiyun 15; Trachsel, Christian 6; Velsko, Irina M.11; Speller, Camilla F.3,16
	2018-07-25
发表期刊	PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
ISSN	0962-8452
卷号	285 期号:1883 页码:10
摘要	Archaeological dental calculus has emerged as a rich source of ancient biomolecules, including proteins. Previous analyses of proteins extracted from ancient dental calculus revealed the presence of the dietary milk protein beta-lactoglobulin, providing direct evidence of dairy consumption in the archaeological record. However, the potential for calculus to preserve other food-related proteins has not yet been systematically explored. Here we analyse shotgun metaproteomic data from 100 archaeological dental calculus samples ranging from the Iron Age to the post-medieval period (eighth century BC to nineteenth century AD) in England, as well as 14 dental calculus samples from contemporary dental patients and recently deceased individuals, to characterize the range and extent of dietary proteins preserved in dental calculus. In addition to milk proteins, we detect proteomic evidence of foodstuffs such as cereals and plant products, as well as the digestive enzyme salivary amylase. We discuss the importance of optimized protein extraction methods, data analysis approaches and authentication strategies in the identification of dietary proteins from archaeological dental calculus. This study demonstrates that proteomic approaches can robustly identify foodstuffs in the archaeological record that are typically under-represented due to their poor macroscopic preservation.
关键词	proteomics dental calculus dietary reconstruction mass spectrometry
DOI	10.1098/rspb.2018.0977
关键词[WOS]	BETA-LACTOGLOBULIN ; SHOTGUN PROTEOMICS ; PROTEIN ; IDENTIFICATION ; MICROFOSSILS ; CONSUMPTION ; EVOLUTION ; CHINA ; FOOD ; FERMENTATION
收录类别	SCI
语种	英语
资助项目	Wellcome Trust[108375/Z/15/Z] ; University of York C2D2 Research Priming Fund grant - Wellcome Trust[097829/Z/11/A] ; White Rose University Consortium collaboration grant ; Max Planck Society ; National Science Foundation[BCS-1523264] ; National Science Foundation[BCS-1516633] ; National Science Foundation[BCS-1643318] ; DNRF Niels Bohr Professorship[DNRF128] ; Oxford University Fell Fund[EBD-10130] ; Kennedy Trust Fund
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Biology ; Ecology ; Evolutionary Biology
WOS记录号	WOS:000439907900020
出版者	ROYAL SOC
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/8807
专题	中国科学院古脊椎动物与古人类研究所
通讯作者	Hendy, Jessica; Speller, Camilla F.
作者单位	1.Max Planck Inst Sci Human Hist, Dept Archaeol, Jena, Germany 2.Max Planck Inst Sci Human Hist, Dept Archaeogenet, Jena, Germany 3.Univ York, Dept Archaeol, BioArCh, York, N Yorkshire, England 4.Univ Oklahoma, Dept Anthropol, Labs Mol Anthropol & Microbiome Res, Norman, OK 73019 USA 5.Univ Zurich, ETH Zurich, Inst Evolutionary Med, Zurich, Switzerland 6.Univ Zurich, ETH Zurich, Funct Genom Ctr, Zurich, Switzerland 7.Univ Oklahoma, Hlth Sci Ctr, Coll Dent, Dept Periodontol, Oklahoma City, OK 73190 USA 8.Univ Copenhagen, Nat Hist Museum Denmark, EvoGen, Copenhagen, Denmark 9.Univ Copenhagen, Fac Hlth & Med Sci, Novo Nordisk Fdn, Ctr Prot Res, Copenhagen, Denmark 10.Univ Oxford, Target Discovery Inst, Discovery Prote Facil, Oxford, England 11.Univ Oxford, Res Lab Archaeol & Hist Art, Palaeogen & Bioarchaeol Res Network, Oxford, England 12.York Osteoarchaeol Ltd, York, N Yorkshire, England 13.Pinellas Dent Specialties, Largo, FL 33776 USA 14.Univ Tennessee, Dept Anthropol, Coll Arts & Sci, Knoxville, TN 37996 USA 15.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origins, Beijing, Peoples R China 16.Univ British Columbia, Dept Anthropol, Vancouver, BC, Canada
推荐引用方式 GB/T 7714	Hendy, Jessica,Warinner, Christina,Bouwman, Abigail,et al. Proteomic evidence of dietary sources in ancient dental calculus[J]. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,2018,285(1883):10.
APA	Hendy, Jessica.,Warinner, Christina.,Bouwman, Abigail.,Collins, Matthew J..,Fiddyment, Sarah.,...&Speller, Camilla F..(2018).Proteomic evidence of dietary sources in ancient dental calculus.PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES,285(1883),10.
MLA	Hendy, Jessica,et al."Proteomic evidence of dietary sources in ancient dental calculus".PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 285.1883(2018):10.

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