Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles

doi:10.7717/peerj.5836

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	Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles
	Milian-Garcia, Yoamel 1,2; Russello, Michael A.2; Castellanos-Labarcena, Jessica 1,2; Cichon, Martin 3; Kumar, Vikas 3,4; Espinosa, Georgina 1; Rossi, Natalia 5; Mazzotti, Frank 6; Hekkala, Evon 7; Amato, George 8; Janke, Axel 3
	2018-11-12
发表期刊	PEERJ
ISSN	2167-8359
卷号	6 页码:16
通讯作者	Milian-Garcia, Yoamel(yoamelmg@gmail.com)
摘要	Four species of true crocodile (genus Crocodylus) have been described from the Americas. Three of these crocodile species exhibit non-overlapping distributions-Crocodylus intermedius in South America, C. moreletii along the Caribbean coast of Mesoamerica, and C. rhombifer confined to Cuba. The fourth, C. acutus, is narrowly sympatric with each of the other three species. In this study, we sampled 113 crocodiles across Crocodylus populations in Cuba, as well as exemplar populations in Belize and Florida (USA), and sequenced three regions of the mitochondrial genome (D-loop, cytochrome b, cytochrome oxidase I; 3,626 base pair long dataset) that overlapped with published data previously collected from Colombia, Jamaica, and the Cayman Islands. Phylogenetic analyses of these data revealed two, paraphyletic lineages of C. acutus. One lineage, found in the continental Americas, is the sister taxon to C. intermedius, while the Greater Antillean lineage is most closely related to C. rhombifer. In addition to the paraphyly of the two C. acutus lineages, we recovered a 5.4% estimate of Tamura-Nei genetic divergence between the Antillean and continental clades. The reconstructed paraphyly, distinct phylogenetic affinities and high genetic divergence between Antillean and continental C. acutus populations are consistent with interspecific differentiation within the genus and suggest that the current taxon recognized as C. acutus is more likely a complex of cryptic species warranting a reassessment of current taxonomy. Moreover, the inclusion, for the first time, of samples from the western population of the American crocodile in Cuba revealed evidence for continental mtDNA haplotypes in the Antilles, suggesting this area may constitute a transition zone between distinct lineages of C. acutus. Further study using nuclear character data is warranted to more fully characterize this cryptic diversity, resolve taxonomic uncertainty, and inform conservation planning in this system.
关键词	Crocodylus acutus American crocodile mtDNA Phylogeny Cryptic species
DOI	10.7717/peerj.5836
关键词[WOS]	COMPLETE MITOCHONDRIAL GENOME ; DNA ; DIVERSITY ; REVEALS ; HYBRIDIZATION ; RHOMBIFER ; SOFTWARE ; SEQUENCE ; POROSUS ; TCS
收录类别	SCI
语种	英语
资助项目	Leibniz Association ; Student Research Assistance Scheme (SRAS) ; IUCN-SSC Crocodile Specialist Group's Student Research Assistance Scheme (SRAS) ; International Foundation for Science[D 5354] ; Idea Wild Organization ; American Museum of Natural History (NY) ; Rufford Foundation for Nature Conservation[19318-B]
项目资助者	Leibniz Association ; Student Research Assistance Scheme (SRAS) ; IUCN-SSC Crocodile Specialist Group's Student Research Assistance Scheme (SRAS) ; International Foundation for Science ; Idea Wild Organization ; American Museum of Natural History (NY) ; Rufford Foundation for Nature Conservation
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000452329500002
出版者	PEERJ INC
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/9124
专题	中国科学院古脊椎动物与古人类研究所
通讯作者	Milian-Garcia, Yoamel
作者单位	1.Univ La Habana, Fac Biol, Dept Bioquim, Havana, Cuba 2.Univ British Columbia, Dept Biol, Kelowna, BC, Canada 3.Senckenberg Gesell Nat Forsch, Senckenberg Biodivers & Climate Res Ctr, Frankfurt, Germany 4.Chinese Acad Sci, Inst Vertebrate Paleontol & Paleoanthropol, Key Lab Vertebrate Evolut & Human Origin, Beijing, Peoples R China 5.Wildlife Conservat Soc, New York, NY USA 6.Univ Florida, Ft Lauderdale Res & Educ Ctr, Ft Lauderdale, FL 33314 USA 7.Fordham Univ, Dept Biol Sci, New York, NY 10023 USA 8.Amer Museum Nat Hist, Sakler Inst Comparat Genom, New York, NY USA
推荐引用方式 GB/T 7714	Milian-Garcia, Yoamel,Russello, Michael A.,Castellanos-Labarcena, Jessica,et al. Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles[J]. PEERJ,2018,6:16.
APA	Milian-Garcia, Yoamel.,Russello, Michael A..,Castellanos-Labarcena, Jessica.,Cichon, Martin.,Kumar, Vikas.,...&Janke, Axel.(2018).Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles.PEERJ,6,16.
MLA	Milian-Garcia, Yoamel,et al."Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles".PEERJ 6(2018):16.