A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)

doi:10.1016/S1002-0160(17)60412-0

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	A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)
	Liu Junchi 1,2,3; Liu Weiguo 1,4; Hu Jing 1
	2018-12-01
发表期刊	PEDOSPHERE
ISSN	1002-0160
卷号	28 期号:6 页码:943-951
摘要	Nitrogen (N) isotope ratio (delta N-15) of soil extractable NO(3)(-)plays a pivotal role in the study of N biogeochemical circulation in ecosystems. However, the NO(3)(-)concentration and its isotope composition of soil samples are unstable, making sample storage critical for preserving the N isotope composition of extracted soil Nevertheless, studies on the appropriate selection of storage methods after soil sampling are scarce. In this study, we compared two commonly used methods for storing soil samples and investigated the stability of N isotopes of soil The results demonstrated that no significant changes in the NO(3)(-)concentration and delta N-15 value occurred in the samples stored at -18 degrees C. However, the soil NO(3)(-)concentration markedly increased, and NO3- delta N-15 value significantly changed after air-drying storage. Meanwhile, we also found that NO3- and its delta N-15 were well preserved in the filtered soil extracts after 1 month. In contrast, the NO(3)(-)concentration gradually decreased and the N-15 in NO(3)(-)was gradually enriched in the bactericidal agent-containing soil mixture solution during the storage period. Overall, our results indicated that N isotopes of NO3- could be effectively preserved in frozen-stored soil samples or filtered soil extracts. For field investigations conducted in remote areas and continued for a long-time period (and lacking a refrigerant supply), soil extraction/filtration using a CaSO4-saturated solution may be a superior preparation and storage method for analyzing N isotopes of soil NO3-.
关键词	air-drying freezing ion exchange isotopic fractionation nitrogen isotope tracing soil extraction soil storage
DOI	10.1016/S1002-0160(17)60412-0
关键词[WOS]	SHORT-TERM CHANGES ; MICROBIAL BIOMASS ; NATURAL-ABUNDANCE ; FOREST ; NITRATE ; PLANT ; NITRIFICATION ; STORAGE ; FRACTIONATION ; DELTA-N-15
收录类别	SCI
语种	英语
资助项目	Key Deployment Project from Chinese Academy of Sciences[KZZD-EW-04-06] ; National Natural Science Foundation of China[41373022] ; National Basic Research Program of China[2013CB955900]
WOS研究方向	Agriculture
WOS类目	Soil Science
WOS记录号	WOS:000456949800010
出版者	SCIENCE PRESS
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.205/handle/311034/9306
专题	中国科学院古脊椎动物与古人类研究所
通讯作者	Liu Weiguo
作者单位	1.Chinese Acad Sci, Inst Earth Environm, State Key Lab Loess & Quaternary Geol, Xian 710075, Shaanxi, Peoples R China 2.Chinese Acad Sci, Key Lab Vertebrate Evolut & Human Origin, Inst Vertebrate Paleontol & Paleoanthropol, Beijing 100044, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Xi An Jiao Tong Univ, Sch Human Settlement & Civil Engn, Xian 710049, Peoples R China
推荐引用方式 GB/T 7714	Liu Junchi,Liu Weiguo,Hu Jing. A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)[J]. PEDOSPHERE,2018,28(6):943-951.
APA	Liu Junchi,Liu Weiguo,&Hu Jing.(2018).A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-).PEDOSPHERE,28(6),943-951.
MLA	Liu Junchi,et al."A Comparison of Different Preservation Methods for Nitrogen Isotopes of Soil Extractable NO3(-)".PEDOSPHERE 28.6(2018):943-951.

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